caa a22 4500 606242929 CHVBK 20210128101321.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s12551-015-0179-1 doi (NATIONALLICENCE)springer-10.1007/s12551-015-0179-1 Optically-controlled platforms for transfection and single- and sub-cellular surgery [Elektronische Daten] [Mark Villangca, Duncan Casey, Jesper Glückstad] Improving the resolution of biological research to the single-cell or sub-cellular level is of critical importance in a wide variety of processes and disease conditions. Most obvious are those linked to aging and cancer, many of which are dependent upon stochastic processes where individual, unpredictable failures or mutations in individual cells can lead to serious downstream conditions across the whole organism. The traditional tools of biochemistry struggle to observe such processes: the vast majority are based upon ensemble approaches analysing the properties of bulk populations, which means that details of individual constituents is lost. What are required, then, are tools with the precision and resolution to probe and dissect cells at the single-micron scale: the scale of the individual organelles and structures that control their function. In this review, we highlight the use of highly-focused laser beams to create systems which provide precise control and specificity at the single-cell or even single-micron level. The intense focal points generated can directly interact with cells and cell membranes, which in conjunction with related modalities such as optical trapping provide a broad platform for the development of single-cell and sub-cellular surgery approaches. These highly tuneable tools have been demonstrated to deliver or remove material from cells of interest, and they can simultaneously excite fluorescent probes for imaging purposes or plasmonic structures for very local heating. We discuss both the history and recent applications of the field, highlighting the key findings and developments over the last 40years of biophotonics research. International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag Berlin Heidelberg, 2015 Optical trapping nationallicence Optoporation nationallicence Transfection nationallicence Single-cell science nationallicence Villangca Mark DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark aut Casey Duncan Engineering and Technology Research Institute, Liverpool John Moores University, Liverpool, UK aut Glückstad Jesper DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark aut Biophysical Reviews Springer Berlin Heidelberg 7/4(2015-12-01), 379-390 1867-2450 7:4<379 2015 7 12551 https://doi.org/10.1007/s12551-015-0179-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12551-015-0179-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Villangca Mark DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Casey Duncan Engineering and Technology Research Institute, Liverpool John Moores University, Liverpool, UK aut NATIONALLICENCE 700 1- Glückstad Jesper DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark aut NATIONALLICENCE 773 0- Biophysical Reviews Springer Berlin Heidelberg 7/4(2015-12-01), 379-390 1867-2450 7:4<379 2015 7 12551