caa a22 4500 606243321 CHVBK 20210128101323.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11694-015-9253-4 doi (NATIONALLICENCE)springer-10.1007/s11694-015-9253-4 Evaluation of in vitro antioxidant and brine shrimp lethality activities of different stem extracts of Zizyphus rugosa Lam [Elektronische Daten] [Md. Hossain, Nizam Uddin, A. Islam, A. Nazmul Hasan, Md. Hossain, Mohammad Hasan, Md. Khalik, Md. Rana] Zizyphus rugosa Lam (Z. rugosa Lam) (Family: Rhamnaceae), has been regarded as a food and medicinal plant. It is locally known as "Bon Boroi” or as "Jongli Boroi” in Bangladesh. Its stem, roots and fruits are used medicinally for the treatment of carbuncle, syphilis, menorrhea, and ulcer tongue. It is also used for dysentery in Laos, Burma, Thailand, China (Hainan, Yunnan), Sri-Lanka and Vietnam. The present study was designed to investigate antioxidant properties (through in vitro method) as well as brine shrimp lethality and phytochemical group evaluation of stem part of Z. rugosa Lam extracted with different solvents i.e. from non polar to polar (petroleum ether>ethyl acetate>ethanol>methanol>water). Phytochemical investigation showed the presence of alkaloids, flavonoid, glycosides and carbohydrates which provides evidence on good to moderate antioxidant and good lethality properties of the subjected plant. Ethyl acetate extract of stem was found to contain the highest amount of phenols (97.188±12.816mg/g gallic acid equivalent) and flavonoids (15.009±0.385mg/g quercetin equivalent). In 2,2-diphenyl,1-picrylhydrazyl (DPPH) free radical scavenging assay, among all the extracts ethanolic stem extract showed the highest scavenging property (IC50 83.550µg/ml) whereas standard drug ascorbic acid showed (IC50 18.348µg/ml). But in nitric oxide (NO) scavenging assay maximum scavenging of NO was found with water extract of stem (IC50 5.975μg/ml) comparatively similar to standard ascorbic acid (IC50 5.934μg/ml). Methanolic stem extract was found to contain the greater reducing power in reducing power capacity assessment (correlation coefficient r=0.99 and P<0.001). In brine shrimp lethality bioassay (BSLB) among all extracts, ZSM part showed good activity (LC50 40.43μg/ml) whereas standard anticancer drug vincristine sulphate showed high toxicity (LC50 2.48μg/ml). The overall findings provide scientific basis for the use of Z. rugosa Lam stem extracts in traditional medicine in the treatment of aforementioned diseases. Hence, the stem may serve as a new potential source of medication. Springer Science+Business Media New York, 2015 Jongli Boroi nationallicence Zizyphus rough nationallicence In vitro antioxidant nationallicence Lethality nationallicence Phytochemicals nationallicence Hossain Md Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut Uddin Nizam Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut Islam A. Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut Nazmul Hasan A. Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut Hasan Mohammad Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut Khalik Md Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut Rana Md Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/3(2015-09-01), 454-462 2193-4126 9:3<454 2015 9 11694 https://doi.org/10.1007/s11694-015-9253-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11694-015-9253-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hossain Md Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut NATIONALLICENCE 700 1- Uddin Nizam Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut NATIONALLICENCE 700 1- Islam A. Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut NATIONALLICENCE 700 1- Nazmul Hasan A. Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut NATIONALLICENCE 700 1- Hasan Mohammad Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut NATIONALLICENCE 700 1- Khalik Md Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut NATIONALLICENCE 700 1- Rana Md Department of Pharmacy, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh aut NATIONALLICENCE 773 0- Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/3(2015-09-01), 454-462 2193-4126 9:3<454 2015 9 11694