caa a22 4500 606243534 CHVBK 20210128101325.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11694-015-9260-5 doi (NATIONALLICENCE)springer-10.1007/s11694-015-9260-5 Analytical method validation and comparison of two extraction techniques for screening of azoxystrobin from widely used crops using LC-MS/MS [Elektronische Daten] [Subhrajit Mukherjee, Subhadeep Mukherjee, Gourab Das, Anjan Bhattacharyya] A simple analytical method was developed and validated in chilli, tomato, grape and mango fruits using liquid chromatography tandem mass spectrometry. The method comprised of extraction with ethyl acetate and cyclohexane mixture followed by d-SPE cleanup employing modified quick, easy, cheap, effective, rugged and safe extraction method and quantified in LC-MS/MS using gradient elution. The method was validated in concentration ranging from 0.01 to 0.1µgg−1. The recovery of azoxystrobin in different crops was ranging from 84.36 to 95.64% at three different concentration levels of analytes with relative standard deviation (HorRat<20%) of 4-14%. The global uncertainty was calculated at limit of quantification level i.e. 0.01µgg−1. In order to evaluate in safety use in India, a field study was conducted with the following extraction method. The calculated half life periods of azoxystrobin were ranging from 3.10-3.46, 3.64-3.46, 1.65-1.96 and 1.32-1.36days respectively for experimental substrates. The PHI values of azoxystrobin in chilli, tomato, grape and mango fruits were determined as 4.76, 3.90, 4.06 and 10.74days respectively. Springer Science+Business Media New York, 2015 Dissipation nationallicence Azoxystrobin nationallicence QuEChERS nationallicence LC-MS/MS nationallicence Validation nationallicence Risk assessment nationallicence Mukherjee Subhrajit Department of Chemistry, Visva Bharati, 731235, Santiniketan, WB, India aut Mukherjee Subhadeep Department of Ag. Chemistry & Soil Science, Calcutta University, 700019, Kolkata, WB, India aut Das Gourab Department of Chemistry, Visva Bharati, 731235, Santiniketan, WB, India aut Bhattacharyya Anjan Department of Ag. Chemicals, Bidhan Chandra Krishi Viswavidyalaya, 741252, Mohanpur, WB, India aut Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/4(2015-12-01), 517-524 2193-4126 9:4<517 2015 9 11694 https://doi.org/10.1007/s11694-015-9260-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11694-015-9260-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mukherjee Subhrajit Department of Chemistry, Visva Bharati, 731235, Santiniketan, WB, India aut NATIONALLICENCE 700 1- Mukherjee Subhadeep Department of Ag. Chemistry & Soil Science, Calcutta University, 700019, Kolkata, WB, India aut NATIONALLICENCE 700 1- Das Gourab Department of Chemistry, Visva Bharati, 731235, Santiniketan, WB, India aut NATIONALLICENCE 700 1- Bhattacharyya Anjan Department of Ag. Chemicals, Bidhan Chandra Krishi Viswavidyalaya, 741252, Mohanpur, WB, India aut NATIONALLICENCE 773 0- Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/4(2015-12-01), 517-524 2193-4126 9:4<517 2015 9 11694