caa a22 4500 606243704 CHVBK 20210128101326.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11694-015-9228-5 doi (NATIONALLICENCE)springer-10.1007/s11694-015-9228-5 Purification and characterization of polygalacturonase from ripened fruits of Musa acuminata cultivar from Kerala ( Musa acuminata cv. Palayankodan) [Elektronische Daten] [Thulasy Gayathri, Ashalatha Nair] Bananas are the fruit crop with strong economic and nutritional relevance. This fruits have a very short shelf life due to the softening of the pulp during ripening. Fruit softening is an important aspect of ripening process in fleshy fruits and is caused by the cumulative action of a group of cell wall modifying enzymes. The present study reports characterization of polygalacturonase (PG) isoforms from ripened fruits of Musa acuminata cv. Palayankodan. Three isoforms of PG was purified by ammonium sulphate fractionation followed by DEAE cellulose ion exchange chromatography and gel filtration using Sephadex G 100. Three isoforms showed two subunits on SDS-PAGE and a single band on native PAGE. Enzyme showed maximum activity at pH 4 (PG1, PG2) and 3.5 (PG3). Fe3+ activated PG1 and PG2, while Ca2+ reduced the activity of all isoforms. Km value for substrate polygalacturonic acid was 0.013, 0.015 and 0.011% for PG1, PG2 and PG3 respectively. Springer Science+Business Media New York, 2015 Polygalacturonase nationallicence Musa acuminata cv. Palayankodan nationallicence Isoforms nationallicence Chromatography nationallicence Km value nationallicence Gayathri Thulasy Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, Kerala, India aut Nair Ashalatha Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, Kerala, India aut Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/2(2015-06-01), 233-239 2193-4126 9:2<233 2015 9 11694 https://doi.org/10.1007/s11694-015-9228-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11694-015-9228-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gayathri Thulasy Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, Kerala, India aut NATIONALLICENCE 700 1- Nair Ashalatha Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, Kerala, India aut NATIONALLICENCE 773 0- Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/2(2015-06-01), 233-239 2193-4126 9:2<233 2015 9 11694