caa a22 4500 606243739 CHVBK 20210128101326.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11694-015-9223-x doi (NATIONALLICENCE)springer-10.1007/s11694-015-9223-x HPTLC-MS based method development and validation for the detection of adulterants in spices [Elektronische Daten] [Roopa Rani, Sharad Medhe, ManMohan Srivastava] Development and validation of high performance thin layer chromatographic method was carried out for the estimation of adulterants (sudan dyes) in turmeric and chili powdered spices. The separation of sudan dyes were obtained over aluminium baked HPTLC plates pre-coated with silica gel RP-18 F254 as stationary phases with optimized triphasic mobile phase acetonitrile: acetone: ammonia (9.0:0.5:0.5; v/v/v) with 5min saturation time. Validation was carried out by testing its specificity, linearity, accuracy, inter and intraday precision, repeatability, limit of detection and limit of quantification according to ICH guidelines. Recording of characteristic molecular ion peaks of sudan I, II, III and IV at 248, 278, 353 and 381, respectively highlights the use of TLC-MS interface confirming the adulteration of sudan dyes in some commercially available powders of spices. Springer Science+Business Media New York, 2015 Adulteration nationallicence Sudan dyes nationallicence HPTLC nationallicence Validation nationallicence TLC-MS nationallicence Rani Roopa Department of Chemistry, Dayalbagh Educational Institute, 282110, Agra, India aut Medhe Sharad Department of Chemistry, Dayalbagh Educational Institute, 282110, Agra, India aut Srivastava ManMohan Department of Chemistry, Dayalbagh Educational Institute, 282110, Agra, India aut Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/2(2015-06-01), 186-194 2193-4126 9:2<186 2015 9 11694 https://doi.org/10.1007/s11694-015-9223-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11694-015-9223-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rani Roopa Department of Chemistry, Dayalbagh Educational Institute, 282110, Agra, India aut NATIONALLICENCE 700 1- Medhe Sharad Department of Chemistry, Dayalbagh Educational Institute, 282110, Agra, India aut NATIONALLICENCE 700 1- Srivastava ManMohan Department of Chemistry, Dayalbagh Educational Institute, 282110, Agra, India aut NATIONALLICENCE 773 0- Journal of Food Measurement and Characterization Springer US; http://www.springer-ny.com 9/2(2015-06-01), 186-194 2193-4126 9:2<186 2015 9 11694