caa a22 4500 606245316 CHVBK 20210128101334.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s12262-012-0725-z doi (NATIONALLICENCE)springer-10.1007/s12262-012-0725-z The Relationship Between Holding Time and the Bacterial Load on Surgical Instruments [Elektronische Daten] [Duygu Percin, Hafize Sav, Hatice Hormet-Oz, Murat Karauz] The aim of this investigation was to determine the bacterial load on used instruments and to evaluate the relationship between the bacterial load and the holding time prior to cleaning. Thirty six sets were evaluated to establish the average number of bacteria per square centimeter. For the experimental study, three different bacteria were prepared in sheep blood and used to contaminate sterile stainless steel pieces with the surface of 10cm2. After incubation at room temperature for 2, 4, 6, 8, 12, 24, 36, and 48h, colonies were counted and compared to time zero. Bacterial counts were between 10 and 250CFU/cm2, depending on the operation site. Bacterial load was found to have increased after 6h. An increase of 3log10 CFU/cm2 was measured after 12h. It is imperative to clean surgical instruments in the first 6h to ensure effective disinfection and sterility. Association of Surgeons of India, 2012 Surgical instruments nationallicence Bacterial load nationallicence Bioburden nationallicence Holding time nationallicence Contamination nationallicence Percin Duygu Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut Sav Hafize Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut Hormet-Oz Hatice Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut Karauz Murat Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut Indian Journal of Surgery Springer India 77/1(2015-02-01), 16-18 0972-2068 77:1<16 2015 77 12262 https://doi.org/10.1007/s12262-012-0725-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12262-012-0725-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Percin Duygu Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut NATIONALLICENCE 700 1- Sav Hafize Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut NATIONALLICENCE 700 1- Hormet-Oz Hatice Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut NATIONALLICENCE 700 1- Karauz Murat Department of Microbiology, Erciyes University, Faculty of Medicine, 38039, Kayseri, Turkey aut NATIONALLICENCE 773 0- Indian Journal of Surgery Springer India 77/1(2015-02-01), 16-18 0972-2068 77:1<16 2015 77 12262