caa a22 4500 606246967 CHVBK 20210128101342.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s12262-013-0855-y doi (NATIONALLICENCE)springer-10.1007/s12262-013-0855-y The Effects of Total Colectomy on Bacterial Translocation in a Model of Acute Pancreatitis [Elektronische Daten] [Rahman Şenocak, Taner Yigit, Zafer Kılbaş, Ali Coşkun, Ali Harlak, Mustafa Menteş, Abdullah Kılıç, Armağan Günal, Orhan Kozak] Prevention of secondary infection is currently the main goal of treatment for acute necrotizing pancreatitis. Colon was considered as the main origin of secondary infection. Our aim was to investigate whether prophylactic total colectomy would reduce the rate of bacterial translocation and infection of pancreatic necrosis. Forty-two Sprague-Dawley rats were used. Pancreatitis was created by ductal infusion of sodium taurocholate. Rats were divided into four groups: group-1, laparotomy + pancreatic ductal infusion of saline; group-2, laparotomy + pancreatic ductal infusion of sodium taurocholate; group-3, total colectomy + pancreatic ductal infusion of saline; and group-4, total colectomy + pancreatic ductal infusion of sodium taurocholate. Forty-eight hours later, tissue and blood samples were collected for microbiological and histopathological analysis. Total colectomy caused small bowel bacterial overgrowth with gram-negative and gram-positive microorganisms. Bacterial count of gram-negative rods in the small intestine and pancreatic tissue in rats with colectomy and acute pancreatitis were significantly higher than in rats with acute pancreatitis only (group-2 versus group-4; small bowel, p = <0.001; pancreas, p = 0.002). Significant correlation was found between proximal small bowel bacterial overgrowth and pancreatic infection (r = 0,836, p = 0.001). In acute pancreatitis, prophylactic total colectomy (which can mimic colonic cleansing and reduction of colonic flora) induces small bowel bacterial overgrowth, which is associated with increased bacterial translocation to the pancreas. Association of Surgeons of India, 2013 Bacterial translocation nationallicence Acute pancreatitis nationallicence Colonic cleansing nationallicence Intestinal flora nationallicence Şenocak Rahman Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut Yigit Taner Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut Kılbaş Zafer Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut Coşkun Ali Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut Harlak Ali Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut Menteş Mustafa Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut Kılıç Abdullah Department of Microbiology, Gülhane Military Medical Academy, Ankara, Etlik, Turkey aut Günal Armağan Department of Pathology, Gülhane Military Medical Academy, Ankara, Etlik, Turkey aut Kozak Orhan Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut Indian Journal of Surgery Springer India 77(2015-12-01), 412-418 0972-2068 77<412 2015 77 12262 https://doi.org/10.1007/s12262-013-0855-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12262-013-0855-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Şenocak Rahman Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut NATIONALLICENCE 700 1- Yigit Taner Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut NATIONALLICENCE 700 1- Kılbaş Zafer Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut NATIONALLICENCE 700 1- Coşkun Ali Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut NATIONALLICENCE 700 1- Harlak Ali Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut NATIONALLICENCE 700 1- Menteş Mustafa Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut NATIONALLICENCE 700 1- Kılıç Abdullah Department of Microbiology, Gülhane Military Medical Academy, Ankara, Etlik, Turkey aut NATIONALLICENCE 700 1- Günal Armağan Department of Pathology, Gülhane Military Medical Academy, Ankara, Etlik, Turkey aut NATIONALLICENCE 700 1- Kozak Orhan Department of General Surgery, Gülhane Military Medical Academy, 06018, Ankara, Turkey aut NATIONALLICENCE 773 0- Indian Journal of Surgery Springer India 77(2015-12-01), 412-418 0972-2068 77<412 2015 77 12262