caa a22 4500 606246991 CHVBK 20210128101343.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s12262-013-0808-5 doi (NATIONALLICENCE)springer-10.1007/s12262-013-0808-5 The Levels of Oxidative Stress Biomarkers in Rats as a Response to Different Techniques of Testicular Biopsy [Elektronische Daten] [S. Resim, E. Kurutas, A. Gul, M. Eren, C. Benlioglu, E. Efe, Y. Atli] Some biopsy techniques may cause oxidative stress. Therefore, we aimed to evaluate the possible role of testicular biopsy-induced damage on production of reactive oxygen species using quantitative and biochemical methods. Adult male Wistar albino rats were randomly divided into five groups. Group 1 (sham) (n = 6) was sham operated. Group 2 (n = 7) underwent fine-needle aspiration testicular biopsy. Group 3 (n = 6) underwent microscopic testicular sperm extraction (micro-TESE). Open testicular biopsy was performed to rats in group 4 (macro-TESE) (n = 8). Group 5 (n = 7) underwent Tru-cut biopsy. Six weeks after the initial operations, orchiectomies were performed. Oxidative stress biomarkers such as superoxide dismutase (SOD) and catalase (CAT) activities, and malondialdehyde (MDA) in biopsy samples were measured as spectrophotometric. Compared with group I, SOD and CAT activities, and MDA levels were elevated significantly in the fine-needle aspiration group, in the macro-TESE group and in the Tru-cut biopsy group (groups II, IV, and V), (p < 0.05). However, there were no significant differences between group I and group III (p > 0.05). These data suggest that micro-TESE biopsy is the best technique among all others. All of the biopsy techniques except micro-TESE may cause the overproduction of reactive oxygen species. We consider that the increased antioxidant enzyme activities (CAT, SOD) may reflect the cellular response against oxidative stress in these groups. Association of Surgeons of India, 2013 Testicular biopsy nationallicence Reactive oxygen species nationallicence Oxidative stress nationallicence Micro-TESE nationallicence Resim S. Department of Urology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Haydarbey mah. Bahceci Hoca Blv. Yesilkent sitesi C Blok Kat 6, Daire 27, 46050, Kahramanmaras, Turkey aut Kurutas E. Department of Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey aut Gul A. Department of Urology, Kahramanmaras State Hospital, 46050, Kahramanmaras, Turkey aut Eren M. Department of Urology, Kahramanmaras State Hospital, 46050, Kahramanmaras, Turkey aut Benlioglu C. Department of Urology, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey aut Efe E. Department of Urology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Haydarbey mah. Bahceci Hoca Blv. Yesilkent sitesi C Blok Kat 6, Daire 27, 46050, Kahramanmaras, Turkey aut Atli Y. Department of Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey aut Indian Journal of Surgery Springer India 77(2015-12-01), 310-313 0972-2068 77<310 2015 77 12262 https://doi.org/10.1007/s12262-013-0808-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12262-013-0808-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Resim S. Department of Urology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Haydarbey mah. Bahceci Hoca Blv. Yesilkent sitesi C Blok Kat 6, Daire 27, 46050, Kahramanmaras, Turkey aut NATIONALLICENCE 700 1- Kurutas E. Department of Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey aut NATIONALLICENCE 700 1- Gul A. Department of Urology, Kahramanmaras State Hospital, 46050, Kahramanmaras, Turkey aut NATIONALLICENCE 700 1- Eren M. Department of Urology, Kahramanmaras State Hospital, 46050, Kahramanmaras, Turkey aut NATIONALLICENCE 700 1- Benlioglu C. Department of Urology, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey aut NATIONALLICENCE 700 1- Efe E. Department of Urology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Haydarbey mah. Bahceci Hoca Blv. Yesilkent sitesi C Blok Kat 6, Daire 27, 46050, Kahramanmaras, Turkey aut NATIONALLICENCE 700 1- Atli Y. Department of Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey aut NATIONALLICENCE 773 0- Indian Journal of Surgery Springer India 77(2015-12-01), 310-313 0972-2068 77<310 2015 77 12262