caa a22 4500 606247203 CHVBK 20210128101344.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s12262-013-0923-3 doi (NATIONALLICENCE)springer-10.1007/s12262-013-0923-3 The Efficacy of Probiotic ( Lactobacillus rhamnosus GG) and 5-ASA (Aminosalicylic Acid) in the Treatment of Experimental Radiation Proctitis in Rats [Elektronische Daten] [Özgür Dandin, Mehmet Akin, Ahmet Balta, Ergün Yücel, Dursun Karakaş, Sezai Demirbaş, Sevim Özdemir, Apdullah Haholu] The aims of the study are to demonstrate the effect of probiotic use on the healing of radiation proctitis (RP) and evaluate the efficiency of fecal biomarkers at follow-up of the treatment. Thirty-two male/female rats were randomly separated into four groups of eight rats. The first group (control) was not radiated. RP was created by 17.5Gy single dose rectal irradiation. The second group (RP) was subjected to RP, but not treated. The third group (RP+ASA) was treated with 5-aminosalicylic acid (5-ASA) 250mg/kg daily by gastric lavage for 14days after the irradiation, and the forth group (RP+LGG) was treated with Lactobacillus GG (LGG) 25 × 100 million CFU daily. Feces samples were taken at the 7th and 14th day of the treatment for fecal biomarkers. Rectums of the rats were resected at the 14th day by laparotomy. Samples were evaluated both macroscopically and microscopically. RP was achieved both macroscopically and microscopically. Weight loss of RP group is statistically significant (p < 0.005) than other groups. The healing ratio of RP+ASA and RP+LGG groups was significantly better than the RP group (p < 0.005) both macroscopically and microscopically. But there was no significant difference between ASA and LGG groups. Biochemically, fecal calprotectin was found to be more effective than fecal myeloperoxidase and fecal lactoferrin to show the efficacy of treatment of radiation proctitis. The results of our study demonstrate that probiotic is as effective as 5-aminosalicylic in the treatment of radiation proctitis, and fecal calprotectin is a useful biomarker in determining the response to the treatment. Association of Surgeons of India, 2013 Radiation proctitis nationallicence Probiotics nationallicence 5-Aminosalicylic acid nationallicence Fecal biomarkers nationallicence Dandin Özgür Department of General Surgery, Bursa Military Hospital, Bursa, Turkey aut Akin Mehmet Department of General Surgery, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut Balta Ahmet Department of General Surgery, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut Yücel Ergün Department of General Surgery, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut Karakaş Dursun Department of General Surgery, Ağrı Military Hospital, Ağrı, Turkey aut Demirbaş Sezai Department of General Surgery, Gülhane Military Medical Academy, Ankara, Turkey aut Özdemir Sevim Department of Radiation Oncology, Istanbul University, Istanbul, Turkey aut Haholu Apdullah Department of Pathology, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut Indian Journal of Surgery Springer India 77(2015-12-01), 563-569 0972-2068 77<563 2015 77 12262 https://doi.org/10.1007/s12262-013-0923-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12262-013-0923-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dandin Özgür Department of General Surgery, Bursa Military Hospital, Bursa, Turkey aut NATIONALLICENCE 700 1- Akin Mehmet Department of General Surgery, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut NATIONALLICENCE 700 1- Balta Ahmet Department of General Surgery, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut NATIONALLICENCE 700 1- Yücel Ergün Department of General Surgery, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut NATIONALLICENCE 700 1- Karakaş Dursun Department of General Surgery, Ağrı Military Hospital, Ağrı, Turkey aut NATIONALLICENCE 700 1- Demirbaş Sezai Department of General Surgery, Gülhane Military Medical Academy, Ankara, Turkey aut NATIONALLICENCE 700 1- Özdemir Sevim Department of Radiation Oncology, Istanbul University, Istanbul, Turkey aut NATIONALLICENCE 700 1- Haholu Apdullah Department of Pathology, Haydarpaşa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey aut NATIONALLICENCE 773 0- Indian Journal of Surgery Springer India 77(2015-12-01), 563-569 0972-2068 77<563 2015 77 12262