caa a22 4500 606247416 CHVBK 20210128101345.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s12262-013-0836-1 doi (NATIONALLICENCE)springer-10.1007/s12262-013-0836-1 Effects of Leptin on Intestinal Ischemia-Reperfusion Injury [Elektronische Daten] [Sevgi Sarsu, Bulent Ozokutan, Mehmet Tarakcioglu, Ibrahim Sarı, Cahit Bağcı] Many clinical conditions such as shock, sepsis, mesenteric thrombosis, necrotizing enterocolitis, and bowel transplantation can cause intestinal ischemia-reperfusion (IR) injury. This study was designed to determine the effects of leptin on intestinal IR injury. Thirty rats were divided into three groups, each containing ten rats: group A (IR group), group B (treatment group), and group C (sham group). After 1h of intestinal ischemia, the clamp was removed in order to perform reperfusion. In group B, 100mg/kg leptin was administered subcutaneously 30min before reperfusion. In groups A and C, 0.1ml physiologic saline was injected. In group A, serum and tissue nitric oxide (NO) levels were significantly decreased, and malondialdehyde levels were significantly increased compared to sham group (p < 0.05). Histopathologic injury was significantly lower in sham group compared to group A. In group B, serum and tissue malondialdehyde levels were significantly decreased (p < 0.05), but serum and tissue NO levels were significantly increased compared to group A (p < 0.05). Histopathologic injury was significantly lower in group B compared to group A (p < 0.05). The results of the present study demonstrated that leptin decreases intestinal IR injury by increasing NO production, rearranging mucosal blood flow, and inhibiting polymorphonuclear leukocyte infiltration. Association of Surgeons of India, 2013 Leptin nationallicence Ischemia nationallicence Reperfusion injury nationallicence Intestine nationallicence Nitric oxide nationallicence Malondialdehyde nationallicence Sarsu Sevgi Department of Pediatric Surgery, Gaziantep Children's Hospital, 27060, Gaziantep, Turkey aut Ozokutan Bulent Department of Pediatric Surgery, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut Tarakcioglu Mehmet Department of Biochemistry, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut Sarı Ibrahim Department of Pathology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut Bağcı Cahit Department of Physiology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut Indian Journal of Surgery Springer India 77(2015-12-01), 351-355 0972-2068 77<351 2015 77 12262 https://doi.org/10.1007/s12262-013-0836-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12262-013-0836-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sarsu Sevgi Department of Pediatric Surgery, Gaziantep Children's Hospital, 27060, Gaziantep, Turkey aut NATIONALLICENCE 700 1- Ozokutan Bulent Department of Pediatric Surgery, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut NATIONALLICENCE 700 1- Tarakcioglu Mehmet Department of Biochemistry, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut NATIONALLICENCE 700 1- Sarı Ibrahim Department of Pathology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut NATIONALLICENCE 700 1- Bağcı Cahit Department of Physiology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey aut NATIONALLICENCE 773 0- Indian Journal of Surgery Springer India 77(2015-12-01), 351-355 0972-2068 77<351 2015 77 12262