Changes in lipid class content and composition of Isochrysis sp. (T-Iso) grown in batch culture
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| 024 | 7 | 0 | |a 10.1007/s10499-015-9884-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10499-015-9884-9 | ||
| 245 | 0 | 0 | |a Changes in lipid class content and composition of Isochrysis sp. (T-Iso) grown in batch culture |h [Elektronische Daten] |c [Tim Nalder, Matthew Miller, Michael Packer] |
| 520 | 3 | |a For decades, the microalgae Isochrysis spp. have been widely utilised as a live feed in aquaculture practices. This species possesses a number of favourable characteristics, notably its long-chain omega-3 polyunsaturated fatty acid (LC n-3 PUFA) content; primarily docosahexaenoic acid (DHA, 22:6n-3). This article describes the lipid class content and composition of this microalga grown in batch culture, covering the entirety of lag, log and stationary growth phases. The total lipid was highest in the lag phase (27pg/cell). Total lipid significantly decreased in the exponential growth (7pg/cell), then steadily increasing for the remainder of growth. The increase in total lipid was due to the accumulation of neutral lipid in the form of triacylglycerides. The DHA content (pg/cell) of the neutral lipid remained relatively unchanged for the duration of growth, with the influx of fatty acids being primarily myristic and palmitic acids. DHA (pg/cell) was found at relatively uniform amounts across all lipid classes. However, the DHA content as a percentage differed greatly between classes. The polar lipid class had a significantly higher DHA content, which peaked at 38% of all polar lipid in log growth. The primary PUFA species present in the glycolipid class was stearidonic acid (18:4n-3). This work gives an overview of the lipid content and composition of Isochrysis sp. (T-Iso) over the entirety of its growth under batch culture. The lipid profile for this species at different stages of culture provides a basal data set that is useful for comparative studies using this organism. | |
| 540 | |a Springer International Publishing Switzerland, 2015 | ||
| 690 | 7 | |a Aquaculture |2 nationallicence | |
| 690 | 7 | |a DHA |2 nationallicence | |
| 690 | 7 | |a FA biosynthesis |2 nationallicence | |
| 690 | 7 | |a Isochrysis affinis galbana (T-Iso) |2 nationallicence | |
| 690 | 7 | |a Microalgae |2 nationallicence | |
| 690 | 7 | |a Omega-3 |2 nationallicence | |
| 690 | 7 | |a ALA : α-linolenic acid |2 nationallicence | |
| 690 | 7 | |a DHA : Docosahexaenoic acid |2 nationallicence | |
| 690 | 7 | |a EPA : Eicosapentaenoic acid |2 nationallicence | |
| 690 | 7 | |a FA : Fatty acid |2 nationallicence | |
| 690 | 7 | |a FAME : Fatty acid methyl ester |2 nationallicence | |
| 690 | 7 | |a FFA : Free fatty acid |2 nationallicence | |
| 690 | 7 | |a FID : Flame ionisation detector |2 nationallicence | |
| 690 | 7 | |a GC : Gas chromatography |2 nationallicence | |
| 690 | 7 | |a GC-MS : Gas chromatography-mass spectrometry |2 nationallicence | |
| 690 | 7 | |a GL : Glycolipid |2 nationallicence | |
| 690 | 7 | |a LC n-3 PUFA : Long-chain omega-3 polyunsaturated fatty acid |2 nationallicence | |
| 690 | 7 | |a MAG : Monoacylglycerol |2 nationallicence | |
| 690 | 7 | |a MUFA : Monounsaturated fatty acid |2 nationallicence | |
| 690 | 7 | |a n-3 : Omega-3 |2 nationallicence | |
| 690 | 7 | |a NL : Neutral lipid |2 nationallicence | |
| 690 | 7 | |a PL : Polar lipid |2 nationallicence | |
| 690 | 7 | |a PUFA : Polyunsaturated fatty acid |2 nationallicence | |
| 690 | 7 | |a SDA : Stearidonic acid |2 nationallicence | |
| 690 | 7 | |a SPE : Solid-phase extraction |2 nationallicence | |
| 690 | 7 | |a TAG : Triacylglycerol |2 nationallicence | |
| 690 | 7 | |a T-Iso : Tahitian isolate |2 nationallicence | |
| 690 | 7 | |a TL : Total lipid |2 nationallicence | |
| 700 | 1 | |a Nalder |D Tim |u Marine Products Team, The New Zealand Institute for Plant & Food Research Limited, 300 Wakefield Quay, Nelson, New Zealand |4 aut | |
| 700 | 1 | |a Miller |D Matthew |u Marine Products Team, The New Zealand Institute for Plant & Food Research Limited, 300 Wakefield Quay, Nelson, New Zealand |4 aut | |
| 700 | 1 | |a Packer |D Michael |u Cawthron Institute, Private Bag 2, Nelson, New Zealand |4 aut | |
| 773 | 0 | |t Aquaculture International |d Springer International Publishing |g 23/5(2015-10-01), 1293-1312 |x 0967-6120 |q 23:5<1293 |1 2015 |2 23 |o 10499 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10499-015-9884-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10499-015-9884-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nalder |D Tim |u Marine Products Team, The New Zealand Institute for Plant & Food Research Limited, 300 Wakefield Quay, Nelson, New Zealand |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Miller |D Matthew |u Marine Products Team, The New Zealand Institute for Plant & Food Research Limited, 300 Wakefield Quay, Nelson, New Zealand |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Packer |D Michael |u Cawthron Institute, Private Bag 2, Nelson, New Zealand |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Aquaculture International |d Springer International Publishing |g 23/5(2015-10-01), 1293-1312 |x 0967-6120 |q 23:5<1293 |1 2015 |2 23 |o 10499 | ||