A transfer function approach for predicting rare cell capture microdevice performance
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 60547981X | ||
| 003 | CHVBK | ||
| 005 | 20210128100412.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150601xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s10544-015-9956-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9956-7 | ||
| 245 | 0 | 2 | |a A transfer function approach for predicting rare cell capture microdevice performance |h [Elektronische Daten] |c [James Smith, Brian Kirby] |
| 520 | 3 | |a Rare cells have the potential to improve our understanding of biological systems and the treatment of a variety of diseases; each of those applications requires a different balance of throughput, capture efficiency, and sample purity. Those challenges, coupled with the limited availability of patient samples and the costs of repeated design iterations, motivate the need for a robust set of engineering tools to optimize application-specific geometries. Here, we present a transfer function approach for predicting rare cell capture in microfluidic obstacle arrays. Existing computational fluid dynamics (CFD) tools are limited to simulating a subset of these arrays, owing to computational costs; a transfer function leverages the deterministic nature of cell transport in these arrays, extending limited CFD simulations into larger, more complicated geometries. We show that the transfer function approximation matches a full CFD simulation within 1.34 %, at a 74-fold reduction in computational cost. Taking advantage of these computational savings, we apply the transfer function simulations to simulate reversing array geometries that generate a "notch filter” effect, reducing the collision frequency of cells outside of a specified diameter range. We adapt the transfer function to study the effect of off-design boundary conditions (such as a clogged inlet in a microdevice) on overall performance. Finally, we have validated the transfer function's predictions for lateral displacement within the array using particle tracking and polystyrene beads in a microdevice. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Rare cell capture |2 nationallicence | |
| 690 | 7 | |a Circulating tumor cell |2 nationallicence | |
| 690 | 7 | |a CTC |2 nationallicence | |
| 690 | 7 | |a Transfer function |2 nationallicence | |
| 690 | 7 | |a Collision dynamics |2 nationallicence | |
| 690 | 7 | |a Cell capture |2 nationallicence | |
| 690 | 7 | |a Design optimization |2 nationallicence | |
| 700 | 1 | |a Smith |D James |u Sibley School of Mechanical and Aerospace Engineering, Cornell University, 14853, Ithaca, NY, USA |4 aut | |
| 700 | 1 | |a Kirby |D Brian |u Sibley School of Mechanical and Aerospace Engineering, Cornell University, 14853, Ithaca, NY, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-11 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9956-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9956-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Smith |D James |u Sibley School of Mechanical and Aerospace Engineering, Cornell University, 14853, Ithaca, NY, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kirby |D Brian |u Sibley School of Mechanical and Aerospace Engineering, Cornell University, 14853, Ithaca, NY, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-11 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | ||