Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion
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| 005 | 20210128100547.0 | ||
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| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6701-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6701-3 | ||
| 245 | 0 | 0 | |a Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion |h [Elektronische Daten] |c [Timothy Turner, Guo-Chang Zhang, Soo Kim, Vijay Subramaniam, David Steffen, Christopher Skory, Ji Jang, Byung Yu, Yong-Su Jin] |
| 520 | 3 | |a Production of lactic acid from renewable sugars has received growing attention as lactic acid can be used for making renewable and bio-based plastics. However, most prior studies have focused on production of lactic acid from glucose despite that cellulosic hydrolysates contain xylose as well as glucose. Microbial strains capable of fermenting both glucose and xylose into lactic acid are needed for sustainable and economic lactic acid production. In this study, we introduced a lactic acid-producing pathway into an engineered Saccharomyces cerevisiae capable of fermenting xylose. Specifically, ldhA from the fungi Rhizopus oryzae was overexpressed under the control of the PGK1 promoter through integration of the expression cassette in the chromosome. The resulting strain exhibited a high lactate dehydrogenase activity and produced lactic acid from glucose or xylose. Interestingly, we observed that the engineered strain exhibited substrate-dependent product formation. When the engineered yeast was cultured on glucose, the major fermentation product was ethanol while lactic acid was a minor product. In contrast, the engineered yeast produced lactic acid almost exclusively when cultured on xylose under oxygen-limited conditions. The yields of ethanol and lactic acid from glucose were 0.31g ethanol/g glucose and 0.22g lactic acid/g glucose, respectively. On xylose, the yields of ethanol and lactic acid were <0.01g ethanol/g xylose and 0.69g lactic acid/g xylose, respectively. These results demonstrate that lactic acid can be produced from xylose with a high yield by S. cerevisiae without deleting pyruvate decarboxylase, and the formation patterns of fermentations can be altered by substrates. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Lactic acid |2 nationallicence | |
| 690 | 7 | |a Xylose |2 nationallicence | |
| 690 | 7 | |a Saccharomyces cerevisiae |2 nationallicence | |
| 690 | 7 | |a Lignocellulose |2 nationallicence | |
| 700 | 1 | |a Turner |D Timothy |u Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 260 Bevier Hall, 61801, Urbana, IL, USA |4 aut | |
| 700 | 1 | |a Zhang |D Guo-Chang |u Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 260 Bevier Hall, 61801, Urbana, IL, USA |4 aut | |
| 700 | 1 | |a Kim |D Soo |u School of Food Science and Biotechnology, Kyungpook National University, Buk-gu, 702-701, Daegu, Korea |4 aut | |
| 700 | 1 | |a Subramaniam |D Vijay |u Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA |4 aut | |
| 700 | 1 | |a Steffen |D David |u Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA |4 aut | |
| 700 | 1 | |a Skory |D Christopher |u Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology (RPT) Research Unit, US Department of Agriculture (USDA), 1815 North University Street, 61604, Peoria, IL, USA |4 aut | |
| 700 | 1 | |a Jang |D Ji |u IT Convergence Materials Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 331-822, Cheonan, Korea |4 aut | |
| 700 | 1 | |a Yu |D Byung |u IT Convergence Materials Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 331-822, Cheonan, Korea |4 aut | |
| 700 | 1 | |a Jin |D Yong-Su |u Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 260 Bevier Hall, 61801, Urbana, IL, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 8023-8033 |x 0175-7598 |q 99:19<8023 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6701-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6701-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Turner |D Timothy |u Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 260 Bevier Hall, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Guo-Chang |u Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 260 Bevier Hall, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Soo |u School of Food Science and Biotechnology, Kyungpook National University, Buk-gu, 702-701, Daegu, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Subramaniam |D Vijay |u Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Steffen |D David |u Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Skory |D Christopher |u Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology (RPT) Research Unit, US Department of Agriculture (USDA), 1815 North University Street, 61604, Peoria, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jang |D Ji |u IT Convergence Materials Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 331-822, Cheonan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Byung |u IT Convergence Materials Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 331-822, Cheonan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jin |D Yong-Su |u Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 260 Bevier Hall, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 8023-8033 |x 0175-7598 |q 99:19<8023 |1 2015 |2 99 |o 253 | ||