One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase
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| 008 | 210128e20150301xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6312-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6312-4 | ||
| 245 | 0 | 0 | |a One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase |h [Elektronische Daten] |c [Julia Marín-Navarro, David Talens-Perales, Julio Polaina] |
| 520 | 3 | |a We describe a simple, efficient process for the production of 6-kestose, a trisaccharide with well-documented prebiotic properties. A key factor is the use of a yeast transformant expressing an engineered version of Saccharomyces invertase with enhanced transfructosylating activity. When the yeast transformant was grown with 30% sucrose as the carbon source, 6-kestose accumulated up to ca. 100g/L in the culture medium. The 6-kestose yield was significantly enhanced (up to 200g/L) using a two-stage process carried out in the same flask. In the first stage, the culture was grown in 30% sucrose at physiological temperature (30°C) to allow overexpression of the invertase. In the second stage, sucrose was added to the culture at high concentration (60%) and the temperature shifted to 50°C. In both cases, 6-kestose was synthesized with high specificity, representing more than 95% of total FOS. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Cell factory |2 nationallicence | |
| 690 | 7 | |a Kestose |2 nationallicence | |
| 690 | 7 | |a Prebiotics |2 nationallicence | |
| 690 | 7 | |a Sucrose |2 nationallicence | |
| 690 | 7 | |a Transglycosylation |2 nationallicence | |
| 700 | 1 | |a Marín-Navarro |D Julia |u Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain |4 aut | |
| 700 | 1 | |a Talens-Perales |D David |u Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain |4 aut | |
| 700 | 1 | |a Polaina |D Julio |u Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2549-2555 |x 0175-7598 |q 99:6<2549 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6312-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6312-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marín-Navarro |D Julia |u Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Talens-Perales |D David |u Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Polaina |D Julio |u Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2549-2555 |x 0175-7598 |q 99:6<2549 |1 2015 |2 99 |o 253 | ||