Efficient mannitol production by wild-type Lactobacillus reuteri CRL 1101 is attained at constant pH using a simplified culture medium
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6730-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6730-y | ||
| 245 | 0 | 0 | |a Efficient mannitol production by wild-type Lactobacillus reuteri CRL 1101 is attained at constant pH using a simplified culture medium |h [Elektronische Daten] |c [Maria Ortiz, Raúl Raya, Fernanda Mozzi] |
| 520 | 3 | |a Mannitol is a natural polyol with multiple industrial applications. In this work, mannitol production by Lactobacillus reuteri CRL 1101 was studied at free- and controlled-pH (6.0-4.8) fermentations using a simplified culture medium containing yeast and beef extracts and sugarcane molasses. The activity of mannitol 2-dehydrogenase (MDH), the enzyme responsible for mannitol synthesis, was determined. The effect of the initial biomass concentration was further studied. Mannitol production (41.5±1.1g/l), volumetric productivity (Q Mtl 1.73±0.05g/l h), and yield (Y Mtl 105±11%) were maximum at pH 5.0 after 24h while the highest MDH activity (1.66±0.09U/mg protein) was obtained at pH 6.0. No correlation between mannitol production and MDH activity was observed when varying the culture pH. The increase (up to 2000-fold) in the initial biomass concentration did not improve mannitol formation after 24h although a 2-fold higher amount was produced at 8h using 1 or 2g cell dry weight/l comparing to the control (0.001g cell dry weight/l). Finally, mannitol isolation under optimum fermentation conditions was achieved. The mannitol production obtained in this study is the highest reported so far by a wild-type L. reuteri strain and, more interestingly, using a simplified culture medium. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Polyol |2 nationallicence | |
| 690 | 7 | |a Lactic acid bacteria |2 nationallicence | |
| 690 | 7 | |a Sugarcane molasses |2 nationallicence | |
| 690 | 7 | |a Fermentation |2 nationallicence | |
| 690 | 7 | |a Sugar alcohol |2 nationallicence | |
| 700 | 1 | |a Ortiz |D Maria |u Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina |4 aut | |
| 700 | 1 | |a Raya |D Raúl |u Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina |4 aut | |
| 700 | 1 | |a Mozzi |D Fernanda |u Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8717-8729 |x 0175-7598 |q 99:20<8717 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6730-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6730-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ortiz |D Maria |u Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Raya |D Raúl |u Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mozzi |D Fernanda |u Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8717-8729 |x 0175-7598 |q 99:20<8717 |1 2015 |2 99 |o 253 | ||