l -Serine overproduction with minimization of by-product synthesis by engineered Corynebacterium glutamicum
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6243-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6243-0 | ||
| 245 | 0 | 0 | |a l -Serine overproduction with minimization of by-product synthesis by engineered Corynebacterium glutamicum |h [Elektronische Daten] |c [Qinjian Zhu, Xiaomei Zhang, Yuchang Luo, Wen Guo, Guoqiang Xu, Jinsong Shi, Zhenghong Xu] |
| 520 | 3 | |a The direct fermentative production of l-serine by Corynebacterium glutamicum from sugars is attractive. However, superfluous by-product accumulation and low l-serine productivity limit its industrial production on large scale. This study aimed to investigate metabolic and bioprocess engineering strategies towards eliminating by-products as well as increasing l-serine productivity. Deletion of alaT and avtA encoding the transaminases and introduction of an attenuated mutant of acetohydroxyacid synthase (AHAS) increased both l-serine production level (26.23g/L) and its productivity (0.27g/L/h). Compared to the parent strain, the by-products l-alanine and l-valine accumulation in the resulting strain were reduced by 87% (from 9.80 to 1.23g/L) and 60% (from 6.54 to 2.63g/L), respectively. The modification decreased the metabolic flow towards the branched-chain amino acids (BCAAs) and induced to shift it towards l-serine production. Meanwhile, it was found that corn steep liquor (CSL) could stimulate cell growth and increase sucrose consumption rate as well as l-serine productivity. With addition of 2g/L CSL, the resulting strain showed a significant improvement in the sucrose consumption rate (72%) and the l-serine productivity (67%). In fed-batch fermentation, 42.62g/L of l-serine accumulation was achieved with a productivity of 0.44g/L/h and yield of 0.21g/g sucrose, which was the highest production of l-serine from sugars to date. The results demonstrated that combined metabolic and bioprocess engineering strategies could minimize by-product accumulation and improve l-serine productivity. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a l -Serine production |2 nationallicence | |
| 690 | 7 | |a Acetohydroxyacid synthase |2 nationallicence | |
| 690 | 7 | |a Transaminases |2 nationallicence | |
| 690 | 7 | |a Corn steep liquor |2 nationallicence | |
| 690 | 7 | |a Corynebacterium glutamicum |2 nationallicence | |
| 700 | 1 | |a Zhu |D Qinjian |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhang |D Xiaomei |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | |
| 700 | 1 | |a Luo |D Yuchang |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | |
| 700 | 1 | |a Guo |D Wen |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | |
| 700 | 1 | |a Xu |D Guoqiang |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | |
| 700 | 1 | |a Shi |D Jinsong |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | |
| 700 | 1 | |a Xu |D Zhenghong |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1665-1673 |x 0175-7598 |q 99:4<1665 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6243-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6243-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhu |D Qinjian |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Xiaomei |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Luo |D Yuchang |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Guo |D Wen |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Guoqiang |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shi |D Jinsong |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Zhenghong |u School of Pharmaceutics Science, Jiangnan University, 214122, Wuxi, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1665-1673 |x 0175-7598 |q 99:4<1665 |1 2015 |2 99 |o 253 | ||