Quantifying viable Vibrio parahaemolyticus and Listeria monocytogenes simultaneously in raw shrimp
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6715-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6715-x | ||
| 245 | 0 | 0 | |a Quantifying viable Vibrio parahaemolyticus and Listeria monocytogenes simultaneously in raw shrimp |h [Elektronische Daten] |c [Zhaohuan Zhang, Haiquan Liu, Yang Lou, Lili Xiao, Chao Liao, Pradeep Malakar, Yingjie Pan, Yong Zhao] |
| 520 | 3 | |a A novel TaqMan-based multiplex real-time PCR method combined with propidium monoazide (PMA) treatment was firstly developed for the simultaneous quantification of viable Vibrio parahaemolyticus and Listeria monocytogenes in raw shrimp. The optimization of PMA concentration showed that 100μM was considered optimal to effectively inhibit 108CFU/mL dead cells of both bacteria. The high specificity of this method was confirmed on tests using 96 target and non-target strains. The optimized assay could detect as low as 101-102CFU/g of each strain on the artificially contaminated shrimp, and its amplification efficiencies were up to 100 and 106% for V. parahaemolyticus and L. monocytogenes, respectively. Furthermore, this assay has been successfully applied to describe the behavior of these two pathogens in raw shrimps stored at 4°C. In conclusion, this PMA TaqMan-based multiplex real-time PCR technique, where the whole procedure takes less than 5h, provides an effective and rapid tool for monitoring contamination of viable V. parahaemolyticus and L. monocytogenes in seafood, improving seafood safety and protecting public health. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a TaqMan-based multiplex real-time PCR |2 nationallicence | |
| 690 | 7 | |a Propidium monoazide |2 nationallicence | |
| 690 | 7 | |a Viable foodborne pathogens |2 nationallicence | |
| 690 | 7 | |a Vibrio parahaemolyticus |2 nationallicence | |
| 690 | 7 | |a Listeria monocytogenes |2 nationallicence | |
| 690 | 7 | |a Raw shrimp |2 nationallicence | |
| 700 | 1 | |a Zhang |D Zhaohuan |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 700 | 1 | |a Liu |D Haiquan |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 700 | 1 | |a Lou |D Yang |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 700 | 1 | |a Xiao |D Lili |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 700 | 1 | |a Liao |D Chao |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 700 | 1 | |a Malakar |D Pradeep |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 700 | 1 | |a Pan |D Yingjie |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 700 | 1 | |a Zhao |D Yong |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/15(2015-08-01), 6451-6462 |x 0175-7598 |q 99:15<6451 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6715-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6715-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Zhaohuan |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Haiquan |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lou |D Yang |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xiao |D Lili |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liao |D Chao |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Malakar |D Pradeep |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pan |D Yingjie |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Yong |u College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, 201306, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/15(2015-08-01), 6451-6462 |x 0175-7598 |q 99:15<6451 |1 2015 |2 99 |o 253 | ||