Effects of 1,25-Dihydroxyvitamin D3 and Vitamin D3 on the Expression of the Vitamin D Receptor in Human Skeletal Muscle Cells
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| 024 | 7 | 0 | |a 10.1007/s00223-014-9932-x |2 doi |
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| 245 | 0 | 0 | |a Effects of 1,25-Dihydroxyvitamin D3 and Vitamin D3 on the Expression of the Vitamin D Receptor in Human Skeletal Muscle Cells |h [Elektronische Daten] |c [Rachele Pojednic, Lisa Ceglia, Karl Olsson, Thomas Gustafsson, Alice Lichtenstein, Bess Dawson-Hughes, Roger Fielding] |
| 520 | 3 | |a Vitamin D receptor (VDR) expression and action in non-human skeletal muscle have recently been reported in several studies, yet data on the activity and expression of VDR in human muscle cells are scarce. We conducted a series of studies to examine the (1) effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on VDR gene expression in human primary myoblasts, (2) effect of 16-week supplementation with vitamin D3 on intramuscular VDR gene expression in older women, and (3) association between serum 25-hydroxyvitamin D (25OHD) and intramuscular VDR protein concentration in older adults. Human primary myoblasts were treated with increasing concentrations of 1,25(OH)2D3 for 18h. A dose-dependent treatment effect was noted with 1nmol/L of 1,25OH2D3 increasing intramuscular VDR mRNA expression (mean fold change±SD 1.36±0.33; P=0.05). Muscle biopsies were obtained at baseline and 16weeks after vitamin D3 supplementation (4,000IU/day) in older adults. Intramuscular VDR mRNA was significantly different from placebo after 16weeks of vitamin D3 (1.2±0.99; −3.2±1.7, respectively; P=0.04). Serum 25OHD and intramuscular VDR protein expression were examined by immunoblot. 25OHD was associated with intramuscular VDR protein concentration (R=0.67; P=0.0028). In summary, our study found VDR gene expression increases following treatment with 1,25OH2D3 in human myoblasts. 25OHD is associated with VDR protein and 16weeks of supplementation with vitamin D3 resulted in a persistent increase in VDR gene expression of vitamin D3 in muscle tissue biopsies. These findings suggest treatment with vitamin D compounds results in sustained increases in VDR in human skeletal muscle. | |
| 540 | |a Springer Science+Business Media New York, 2014 | ||
| 690 | 7 | |a Vitamin D |2 nationallicence | |
| 690 | 7 | |a Skeletal muscle |2 nationallicence | |
| 690 | 7 | |a Vitamin D receptor |2 nationallicence | |
| 690 | 7 | |a Human |2 nationallicence | |
| 690 | 7 | |a Primary myoblasts |2 nationallicence | |
| 690 | 7 | |a Aging |2 nationallicence | |
| 700 | 1 | |a Pojednic |D Rachele |u Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA |4 aut | |
| 700 | 1 | |a Ceglia |D Lisa |u Division of Endocrinology, Diabetes and Metabolism, Tufts Medical Center, Boston, MA, USA |4 aut | |
| 700 | 1 | |a Olsson |D Karl |u Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden |4 aut | |
| 700 | 1 | |a Gustafsson |D Thomas |u Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden |4 aut | |
| 700 | 1 | |a Lichtenstein |D Alice |u Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA |4 aut | |
| 700 | 1 | |a Dawson-Hughes |D Bess |u Bone Metabolism Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA |4 aut | |
| 700 | 1 | |a Fielding |D Roger |u Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA |4 aut | |
| 773 | 0 | |t Calcified Tissue International |d Springer US; http://www.springer-ny.com |g 96/3(2015-03-01), 256-263 |x 0171-967X |q 96:3<256 |1 2015 |2 96 |o 223 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00223-014-9932-x |q text/html |z Onlinezugriff via DOI |
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| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
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| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00223-014-9932-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pojednic |D Rachele |u Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ceglia |D Lisa |u Division of Endocrinology, Diabetes and Metabolism, Tufts Medical Center, Boston, MA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Olsson |D Karl |u Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gustafsson |D Thomas |u Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lichtenstein |D Alice |u Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dawson-Hughes |D Bess |u Bone Metabolism Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fielding |D Roger |u Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Calcified Tissue International |d Springer US; http://www.springer-ny.com |g 96/3(2015-03-01), 256-263 |x 0171-967X |q 96:3<256 |1 2015 |2 96 |o 223 | ||