Increased Endoplasmic Reticulum Stress in Mouse Osteocytes with Aging Alters Cox-2 Response to Mechanical Stimuli
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| 024 | 7 | 0 | |a 10.1007/s00223-014-9944-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00223-014-9944-6 | ||
| 245 | 0 | 0 | |a Increased Endoplasmic Reticulum Stress in Mouse Osteocytes with Aging Alters Cox-2 Response to Mechanical Stimuli |h [Elektronische Daten] |c [Sreeda Chalil, Richard Jaspers, Ralph Manders, Jenneke Klein-Nulend, Astrid Bakker, Louise Deldicque] |
| 520 | 3 | |a Aging reduces bone mass as well as the anabolic response of bone to mechanical stimuli, resulting in osteopenia. Endoplasmic reticulum (ER) stress impairs the response of myogenic cells to anabolic stimuli, and is involved in sarcopenia, but whether ER stress also contributes to osteopenia is unknown. Therefore, we tested whether ER stress exists in bones of aged mice, and whether this impairs the osteocyte response to mechanical stimulation. Primary osteocytes were obtained from long bones of adult (8months) and old (24-26months) mice, treated with or without the pharmacological ER stress inducer tunicamycin, and either or not subjected to mechanical loading by pulsating fluid flow (PFF). The osteocyte response to PFF was assessed by measuring cyclooxygenase-2 (Cox-2) mRNA levels and nitric oxide (NO) production. mRNA levels of ER stress markers were higher in old versus adult osteocytes (+40% for activating transcription factor-4, +120% for C/EBP homologous protein, and +120% for spliced X-box binding protein-1, p<0.05). The Cox-2 response to PFF was fourfold decreased in cells from old bones (p<0.001), while tunicamycin decreased PFF-induced Cox-2 expression by threefold in cells from adult bones (p<0.01). PFF increased NO production by 50% at 60min in osteocytes from old versus adult bones (p<0.01). In conclusion, our data indicate that the expression of several ER stress markers was higher in osteocytes from bones of old compared to adult mice. Since ER stress altered the response of osteocytes to mechanical loading, it could be a novel factor contributing to osteopenia. | |
| 540 | |a Springer Science+Business Media New York, 2014 | ||
| 690 | 7 | |a ER stress |2 nationallicence | |
| 690 | 7 | |a Unfolded protein response |2 nationallicence | |
| 690 | 7 | |a Nitric oxide |2 nationallicence | |
| 690 | 7 | |a Cyclooxygenase-2 |2 nationallicence | |
| 690 | 7 | |a Osteopenia |2 nationallicence | |
| 690 | 7 | |a Tunicamycin |2 nationallicence | |
| 690 | 7 | |a Pulsating fluid flow |2 nationallicence | |
| 700 | 1 | |a Chalil |D Sreeda |u Exercise Physiology Research Group, Department of Kinesiology, KU Leuven, Tervuursevest 101, Box 1500, 3001, Louvain, Belgium |4 aut | |
| 700 | 1 | |a Jaspers |D Richard |u Laboratory for Myology, Move Research Institute Amsterdam, Faculty of Human Movement Sciences, VU University Amsterdam, Van der Boechorststraat 9, 1081, Amsterdam, The Netherlands |4 aut | |
| 700 | 1 | |a Manders |D Ralph |u Department of Nutritional Sciences, Faculty of Health and Medical Sciences, University of Surrey, Duke of Kent Building, Guildford, GU2 7TE, Surrey, UK |4 aut | |
| 700 | 1 | |a Klein-Nulend |D Jenneke |u Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), MOVE Research Institute Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahler Laan 3004, 1081, Amsterdam, The Netherlands |4 aut | |
| 700 | 1 | |a Bakker |D Astrid |u Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), MOVE Research Institute Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahler Laan 3004, 1081, Amsterdam, The Netherlands |4 aut | |
| 700 | 1 | |a Deldicque |D Louise |u Exercise Physiology Research Group, Department of Kinesiology, KU Leuven, Tervuursevest 101, Box 1500, 3001, Louvain, Belgium |4 aut | |
| 773 | 0 | |t Calcified Tissue International |d Springer US; http://www.springer-ny.com |g 96/2(2015-02-01), 123-128 |x 0171-967X |q 96:2<123 |1 2015 |2 96 |o 223 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00223-014-9944-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00223-014-9944-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chalil |D Sreeda |u Exercise Physiology Research Group, Department of Kinesiology, KU Leuven, Tervuursevest 101, Box 1500, 3001, Louvain, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jaspers |D Richard |u Laboratory for Myology, Move Research Institute Amsterdam, Faculty of Human Movement Sciences, VU University Amsterdam, Van der Boechorststraat 9, 1081, Amsterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Manders |D Ralph |u Department of Nutritional Sciences, Faculty of Health and Medical Sciences, University of Surrey, Duke of Kent Building, Guildford, GU2 7TE, Surrey, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Klein-Nulend |D Jenneke |u Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), MOVE Research Institute Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahler Laan 3004, 1081, Amsterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bakker |D Astrid |u Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), MOVE Research Institute Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahler Laan 3004, 1081, Amsterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Deldicque |D Louise |u Exercise Physiology Research Group, Department of Kinesiology, KU Leuven, Tervuursevest 101, Box 1500, 3001, Louvain, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Calcified Tissue International |d Springer US; http://www.springer-ny.com |g 96/2(2015-02-01), 123-128 |x 0171-967X |q 96:2<123 |1 2015 |2 96 |o 223 | ||